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KMID : 0613820120220081046
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Journal of Life Science
2012 Volume.22 No. 8 p.1046 ~ p.1051
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Effect of 18 Irradiation on Neurotransmitters in the Brains of Goldfish Carassius auratus
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Park Nam-Gyu
Go Hye-Jin
Kim Gun-Do
Lee Jong-Kyu
Kil Sang-Hyeong
Lee Byung-Woo
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Abstract
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In order to investigate the changes in bioactive materials induced in goldfish brains by 18F irradiation, the variations in the neurotransmitter levels in the whole brain were studied. The distance between the goldfish and 580 mCi of 18F was about 4 cm, and the exposure lasted for 4 hrs. The absorption level calculated based on the distance, exposure time, and half-life of 18F was approximately 2 Gy. After sacrifice by 18F irradiation or untreated conditions, ten brains were dissected or immediately frozen, respectively. The tissues were extracted in acetic acid. After lyophilization, the samples were dissolved in distilled water and were further purified on a reverse-phase HPLC column. There were no differences in the intensities of the bioactive materials between 18F-exposed goldfish and control goldfish, while the only peak corresponded to 13 min, which indicated a significant increase in the irradiated brains. Our analysis has found that this compound is tryptophan. This result suggests that 18F leads to changes in a classical neurotransmitter, tryptophan, in both the brains of control goldfish and goldfish contaminated by irradiation.
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KEYWORD
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Irradiation, goldfish brain, tryptophan, HPLC
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